<p><b>Copyright information:</b></p><p>Taken from "The presence of β-adrenoceptors sensitizes α-adrenoceptors to desensitization after chronic epinephrine treatment"</p><p>http://www.biomedcentral.com/1471-2210/7/16</p><p>BMC Pharmacology 2007;7():16-16.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2234403.</p><p></p>o αand α) and αC4 (corresponding to α) gene products (Table 3). The reactions amplified fragments of the expected size from each set of primers. αC10/C4 primers amplified 233 bp products from SH-SY5Y mRNA that were sensitive to digestion by II (specific for the αproduct). Restriction digestion with XI of the 630 bp product of α 2C4 primer amplification gave three fragments of 271, 225 and 78 bp. All reactions were performed in the presence (+) or absence (-) of reverse transcriptase (RT) to rule out the possibility of DNA contamination. Lane M designates the 100 bp ladder; the 500 bp fragment is indicated by an arrow in each panel