YFP-fused Gag proteins were expressed in 293T cells for twenty-four hours. VLPs in the cell supernatant were purified. Cell lysates and VLPs were analyzed by Western blotting using anti (α)-GFP antibodies. Anti (α)-GAPDH antibodies were used as loading controls. This figure is representative of three independent experiments. 293T cells were transfected with constant amounts of pCMV-Pr55-luc and increasing amounts of wild type or mutated YFP-fused Gag expressors. Twenty-four hours post-transfection, cells were collected and BRET ratios determined. BRET ratios are plotted in function of their corresponding total YFP/luc ratio. This figure is representative of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "The host protein Staufen1 interacts with the Pr55zinc fingers and regulates HIV-1 assembly via its N-terminus"</p><p>http://www.retrovirology.com/content/5/1/41</p><p>Retrovirology 2008;5():41-41.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2409373.</p><p></p
