Human corneal fibroblasts were induced with the mixture of PDGF (AA, AB, and BB) at a final concentration of 20 ng/ml for 1 h or 8 h in the presence or absence of 100 μM AG-490. Protein levels were measured by immunoblotting using a polyclonal STAT3 antibody. PDGF treatment significantly increased STAT3 protein expression by 2.5 fold (1 h) to 2.96 fold (8 h). β-Actin immunoblot shows the equal loading in each lane.<p><b>Copyright information:</b></p><p>Taken from "PDGF-driven proliferation, migration, and IL8 chemokine secretion in human corneal fibroblasts involve JAK2-STAT3 signaling pathway"</p><p></p><p>Molecular Vision 2008;14():1020-1027.</p><p>Published online 30 May 2008</p><p>PMCID:PMC2408775.</p><p></p
