P1-luc and Stau1-YFP expressors. 48 hours post-transfection, Stau1-YFP and Stau1-YFP contents in the cells were analyzed by Western blotting using anti (α)-GFP antibodies. 293T cells were transfected with constant amounts of CA-p2-NC-p1-Rluc-expressing plasmid and increasing amounts of wild-type or mutated YFP-fused Stau1 expressors. Twenty four hours post-transfection, live transfected cells were used for CA-p2-NC-p1/Stau1 BRET assays. BRET ratios are plotted in function of their corresponding total YFP/luc ratio. n = 4. BRET ratios were compared at identical total YFP/luc ratio and corrected by subtracting the background BRET ratio calculated for unfused YFP and CA-p2-NC-p1-luc co-expression. The corrected BRET ratio between CA-p2-NC-p1-luc and Stau1-YFP was arbitrarily set to 100%. n = 4. 293T cells were co-transfected with Pr55and wild-type or N-terminally truncated Stau1-Flag expressors. Twenty-four hours post-transfection, cell extracts were prepared and subjected to immunoprecipitation using anti-Flag antibodies. Cell lysates and immune complexes were analyzed by Western blotting using anti (α)-Flag and anti (α)-CA antibodies. Anti (α)-GAPDH antibodies were used as a loading control. n = 2.<p><b>Copyright information:</b></p><p>Taken from "The host protein Staufen1 interacts with the Pr55zinc fingers and regulates HIV-1 assembly via its N-terminus"</p><p>http://www.retrovirology.com/content/5/1/41</p><p>Retrovirology 2008;5():41-41.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2409373.</p><p></p
