(A) Analysis of mRNA expression by semi–qRT-PCR in human mammary epithelial cells (HMEC) and human umbilical vein epithelial cells (HUVEC) expressing constitutively active Notch1 (Notch1ICD) or Notch4 (Notch4ICD)
(B) Analysis of mRNA expression by qRT-PCR in HMEC expressing Notch1ICD. Results are normalized to the vector control ( = 3). *, P < 0.05. Error bars show SEM. (C) Immunoblots for Slug and Snail in HMEC, HUVEC, and human aortic endothelial cells (HAEC) transduced with Notch1ICD, Notch4ICD, or the empty vector. (D) HMEC expressing Jagged1 or Dll4 were cocultured with parental HMEC and immunoblotting for Slug was performed.<p><b>Copyright information:</b></p><p>Taken from "Slug is a direct Notch target required for initiation of cardiac cushion cellularization"</p><p></p><p>The Journal of Cell Biology 2008;182(2):315-325.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2483533.</p><p></p
