Expression of the reporter gene was detected by RT-PCR using DNase I digested RNA extracted from transgenic worms as template

Abstract

For the negative controls (RNA-PCR) RT-PCR was performed using the same template without adding reverse transcriptase.<p><b>Copyright information:</b></p><p>Taken from "analysis of noncoding RNA promoter motifs"</p><p>http://www.biomedcentral.com/1471-2199/9/71</p><p>BMC Molecular Biology 2008;9():71-71.</p><p>Published online 5 Aug 2008</p><p>PMCID:PMC2527325.</p><p></p