(A) PAE cells stably expressing GFP-Rac1 at levels below endogenous were synchronized in G1/S by serum starvation followed by hydroxyurea and then released
The percentage of cells with nuclear Rac1 was determined hourly and plotted as mean ± SEM ( = 4). (B) Aliquots of the cells analyzed in A were scraped from plates at the indicated times and analyzed for stage of the cell cycle by propidium iodide and cytofluorimetry. (C) Unsynchronized COS-1 cells were transfected with GFP-Rac1 and, after 16 h, fixed and stained for cyclin A. Although the cell expressing GFP-Rac1 in the nucleus (arrows) stained for cyclin A, a marker of G2/M, those excluding the protein from the nucleus (arrowheads) did not. This correlation held for 93% of transfected cells examined (>100). Bars, 10 μm.<p><b>Copyright information:</b></p><p>Taken from "Rac1 accumulates in the nucleus during the G2 phase of the cell cycle and promotes cell division"</p><p></p><p>The Journal of Cell Biology 2008;181(3):485-496.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2364699.</p><p></p
