Percent stenosis calculated from UBM parameters correlates with histological measurements.

Abstract

<p>(a) Transverse B-mode image of RCCA 2 and 9 weeks after surgery. Upper Images, Adventitia is marked “A”, plaque “P”, “Medial layer M”. Lower, images are identical, but the inner elastic lamina is outlined with dotted white line, lumen with a red dotted line. Trichrome-stained transverse sections of the same 9 wk artery; internal elastic lamina (IEL) and lumen are outlined in white and red, respectively. (b) IMT measurements of casted and cuffed animals after 2 and 9 weeks of surgery. n.s. Not significant. (c) Linear regression analysis of percent stenosis estimated by UBM and histology. See Materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029944#s2" target="_blank">Methods</a> for calculations of percent stenosis. Dotted lines indicate 95% confidence intervals; correlation coefficient = 0.75, n = 10 casts. (d) Comparison of UBM and histology measurements using Bland-Altman analysis. Dotted lines indicate 95% limits of agreement. Bias is indicated by solid line, was calculated as −4.76 mm±13.3 SD.</p