<p>B: Dose-dependent inhibition of the <i>Nab1</i> gene in the Luciferase Assay after transfection of MCF7 cells with miR-709. C: Luciferase Assay with pFN4 (3′UTR <i>Eps15</i>) and miR-186. The first bar demonstrates relative level of luciferase activity after transfection of MCF-7 cells with pFN4 only. Second bar: pFN4 + miR-186. Third bar: pFN4 mut + miR-186. Asterisks represent statistical significance (p<0.001). D: Luciferase Assay with pFN7 (3′UTR <i>Nab1</i>) and miR-709. The first bar demonstrates relative level of luciferase activity after transfection of MCF-7 cells with pFN7 only. Second bar: pFN7 + miR-709. Third bar: pFN7 mut + miR-709. Bars represent the normalized average of relative luciferase units.</p