Occludin-labels are associated with TJ and non-TJ structures.

Abstract

<p>(A) A projection image of deeper layers of the same area of the epidermal sheet shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031641#pone-0031641-g001" target="_blank">Figure 1D</a>. (A′-A″″) 3D images of the third (3), fourth (4) and fifth (5) cells from the uppermost nucleated cell layers of the square in A. (B, C) Occludin immunoelectron microscopy. The labels are associated with TJs (brackets) in SG1 and SG2, but with small dots on the cell membrane in the lower epidermis (C″) (arrow). Silver intensified nanogold labels (B) and 5 nm-sized gold labels (C). B′, C′ and C″ are high-magnification views of B and C. Bars = 10 µm (A), 500 nm (B, C) and 200 nm (B′, C′, and C″).</p