Abstract

<p>MIMS image of mouse stereocilia <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030576#pone.0030576-Zhang2" target="_blank">[24]</a> from an 8×8×2-µm volume in 256×256×90 voxels (acquisition time 1966 minutes). Renderings of <sup>12</sup>C<sup>14</sup>N (A), <sup>12</sup>C<sup>15</sup>N/<sup>12</sup>C<sup>14</sup>N HSI (B) both rendered in ImageVis3D <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030576#pone.0030576-ImageVis3D1" target="_blank">[25]</a>. The scale bar in (B) ranges from the natural ratio to the value in the <sup>15</sup>N-enriched chow, which corresponds to the maximum ratio that could be reached in newly synthesized protein (multiplied by 10000). Regions of high turnover (solid arrow), medium turnover (double arrow), and low turnover (outlined arrow) are clearly visible in (B). The resulting segmentation (C) is rendered in Seg3D <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030576#pone.0030576-Seg3D1" target="_blank">[26]</a> and these same classes of high, medium, and low turnover are shown colored as red, green, and blue respectively.</p

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