Multilayered TJ-protein immunoreactive networks in the human epidermis.

Abstract

<p>(A, A′) Biotin-tracer passed through some occludin-positive dots (arrows), but stopped at the most superficial dots (arrowhead). (B, B′) The CDSN-positive SG1 and SG2 have occludin-positive dots (arrows). (C, D) Occludin immunostaining of a sweat duct (C) and an epidermal sheet (D). D and D″ show a projection image of the upper three nucleated cell layers. D″ shows a 3D image from the corner (arrow). D′″ shows slice views dissecting uppermost (1), the second (2) and the third (3) cells. (E, F) EM of TJs (brackets) in the sweat duct (E) and the epidermis (F). A′, B′ and D′ are high-magnification views of A, B and D respectively. Bars = 10 µm (A–D′) and 200 nm (E, F).</p