MyoE localization.

Abstract

<p>A–F are images of the same field and are maximum intensity projections of a Z-series stack. A–C show the co-localization of MyoE-GFP and mCherry-SynA at the Spitzenkörper (arrows). SynA localizes to the Spitzenkörper and to the plasma membrane near the apex (B). In D–F, the thresholds are chosen to reveal the punctate staining in the hypha while overexposing the MyoE-GFP and mCherry-SynA at the hyphal tip. MyoE-GFP localizes to numerous small puncta and some larger structures that may be endosomes (e.g. arrow). G. Faint localization of MyoE-GFP at forming septa (arrows). H. A three-dimensional projection of a hyphal tip showing MyoE-GFP and mCherry-SynA. Although MyoE and SynA co-localize at the Spitzenkörper, many puncta behind the tip show GFP fluorescence or mCherry fluorescence, but it was not clear that there was any obligate co-localization.</p