Fluorescence recovery after photobleaching (FRAP) of GFP-SynA in myoE+ and myoEΔ strains.

Abstract

<p>The tips of the two strains were photobleached at T = 0 (sec). In the <i>myoE</i>+ strain recovery is rapid. GFP-SynA appears at the tip within 30 sec of photobleaching and quickly localizes to the Spitzenkörper (arrows). This indicates that vesicles with SynA move rapidly to the tip and move through the Spitzenkörper before fusing with the plasma membrane. In the <i>myoE</i>Δ strain, the GFP-SynA is also visible at the tip at 30 sec after bleaching. MYOE, thus is not required for movement of GFP-SynA-containing vesicles to the tip. The GFP-SynA does not go through the Spitzenkörper, moreover, but fuses with the plasma membrane in a broad region of the tip.</p