Decreased H4R expression in GCs.

Abstract

<p>(A) Representative blots of HRH4 expression in normal mucosa and gastric tumor tissues. α-tubulin was used as a stable endogenous control. (B) The histogram shows the anlysis of the results from the immunoblottings. The relative expression value of HRH4 protein (normalized by α-tubulin) in GCs is expressed as an average ratio±s.e. of tumor dose to matched adjacent normal tissue dose. *p<0.01 vs. Control, ANT. (C) Real-time PCR assay was carried out as described under <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031207#s2" target="_blank">Materials and Methods</a> section, boxplots of relative HRH4 mRNA(HRH4/GAPDH) measured with real-time PCR analysis showing median; box: 25th–75th percentile; bars: largest and smallest values within 1.5 box lengths; little cross: outliers. The results were obtained from 3 reactions in each sample. (D) Representative immunofluorescent microscope analysis of paired samples of GC tissue and adjacent normal tissue using anti human HRH4 monoclonal antibody (red). Nuclei were stain with DAPI (blue). Sample I: gastric cancer (GC); sample II: adjacent normal control (ANT). Arrows point to region of positive staining.</p