NKG2D and LFA-1 act synergistically in mediating NK cell degranulation.

Abstract

<p>NK and CD4⁺ T cells were activated for 4 days <i>in vitro</i> as described, and co-cultured for 4 hours with FITC conjugated anti-CD107a+anti-CD107b mAbs. 10 ug/mL blocking antibodies (or relevant isotype-matched control Ig) were added where indicated. Flow cytometry was performed to assess degranulation of (A) CD56^dim and (B) CD56^bright NK cells. (C) CD56^dim NK cells were gated based on surface expression of CD94/NKG2A, and degranulation of each subset was evaluated. * <i>P</i><0.05.</p