<p>IFN-γ levels after <i>in vitro</i> restimulation of splenocytes from Ag19 kDa immunized mice. As APC we used AEC (AEC<sub>19 kDa</sub>) and PuM (PuM<sub>19 kDa</sub>) pulsed with Ag19 kDa as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032125#s4" target="_blank">Materials and Methods</a>. One week after the last immunization with Ag19 kDa, mice were sacrificed and splenocytes from immunized and unimmunized mice were co-cultured with AEC<sub>19 kDa</sub> and PuM<sub>19 kDa</sub> for 72 h. Data are expressed as mean ± SD from 3 mice per group. A representative of two independent experiments is shown. * represents levels significantly different from unimmunized control, p<0.05.</p

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