Replication of HIV-1 Nef chimeras in U87MG-CD4⁺/CXCR4⁺ cells.

Abstract

<p>A) U87MG- CD4⁺/CXCR4⁺ cells (2×10⁴ per well of a 96-well plate) were infected with 200 pg p24 equivalents/ml of wild-type HIV-1 NL4-3, a Nef-defective mutant (ΔNef), or the indicated Nef chimeras in a final culture volume of 200 µl. HIV p24 levels were determined by ELISA 5 days later. Data are presented as percent of p24 release observed relative to the HIV-1 NL4-3 control ± S.D. B) U87MG-CD4⁺CXCR4⁺ cells (1×10⁵ per well of a 6-well plate) were infected with 1 ng p24 equivalents/ml of the same panel of viruses as in part A. Viral Nef and gag protein expression was verified by immunoblotting lysates of infected cells 4 days later. Lysates from uninfected cells are included as a negative control (U87MG; far left lane).</p