Inhibition of axonal trafficking in neurons from PS1 and APP/PS1 mice is not specific to mitochondria.

Abstract

<p><b>A.</b> Analysis of real time movement of round-shaped vesicles (endosomes or lysosomes, “v”) that were not stained with TMRM was done using same movies that were acquired to study mitochondrial (‘m’) trafficking. Scale bar, 4 µm. <b>B.</b> Similar to mitochondria, round-shaped vesicles move with reduced velocities in both, anterograde and retrograde directions, travel shorter distances between stops in neurons from APP, PS1 and APP/PS1 mice (<b>C</b>), and stop more frequently in neurons from PS1 and APP/PS1 mice (<b>D</b>). Analysis was done in randomly selected neurons (13–19 for every genotype); pattern and rate of motion of 10 to 58 individual vesicles was analyzed. *p<0.001.</p