Flow cytometry analysis of freshly isolated AEC from mouse lungs.


<p>Total lung cells were obtained by using Corti's protocol with some modifications as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032125#s4" target="_blank">Material and Methods</a>. Leukocytes were first depleted with anti-CD45 microbeads and subsequently, the CD45<b><sup>−</sup></b> cells were depleted of contaminating endothelial cells using anti-CD146 microbeads. The remaining CD45<sup>−</sup>CD146<sup>−</sup> cells were considered to be AEC. These cells were fixed and stained intracellularly with antibodies to CD74 (AEC II marker) and podoplanin (T1α) (AEC I marker). A representative dot plot of flow cytometry analysis of CD74 and T1α expression in freshly isolated AEC from three independent experiments is shown. Percentage numbers represent gated cells from total cells.</p

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