Inhibitory dose-response curve of AL-9 for purified PI4KIIIα and PI4KIIIβ.
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Abstract
<p>The enzymes were preincubated for 10 min with the indicated concentrations of AL-9 or DMSO and the reaction was started by addition of 100 µM ATP and 150 µM PI∶PS substrate as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002576#s4" target="_blank">Materials and Methods</a>. Activity, measured as conversion of ATP to ADP, is expressed as percent of the DMSO control. Shown is a representative experiment of three independent experiments performed in duplicate. IC<sub>50</sub> and SD of PI4KIIIα and PI4KIIIβ are indicated.</p
