Expression of NleH-GFP upon <i>E. coli</i> O157:H7 ZAP193 contact with EBL cells.

Abstract

<p>ZAP193 transformed with plasmids expressing GFP constitutively (pAJR145; <i>rpsm</i>::<i>gfp</i>) or translational fusions of <i>nleH</i> or <i>tir</i> to <i>gfp</i> under the control of their native promoter (pAHE8; NleH1-GFP, pAHE22; NleH2-GFP, pAJR75; Tir-GFP) were added to EBL cells and incubated for 0, 5, 30, 60 or 180 minutes at 37°C, 5% CO₂ before the removal of supernatant and fixation of cells with 4% paraformaldehyde. The panel of images is representative of all time points tested, apart from Tir-GFP, that showed strong early expression during cell contact but was markedly reduced at 180 minutes.</p