Quantitative PCR of NleH transcripts in LEE regulator knockouts.

Abstract

<p>RNA was collected from ZAP193 strains WT, Δler and ΔgrlA grown to OD₆₀₀ = 1.2 in MEM and cDNA prepared. NleH1, NleH2, GapA, Tir and 16S RNA transcript was then quantified by q-PCR, NleH values normalised to that of 16S RNA, and the fold change calculated comparing mutant to wild-type. Bars represent the average of three biological samples. Error bars represent the standard error of the mean.</p