NF-κB activity in the presence of NleH variants.

Abstract

<p>HEK293T cells were co-transfected with a luciferase reporter plasmid under the control of consensus κB sites, a β-galactosidase plasmid and a control (pCMV), NleH or OspG vector. After 40 hours, cells were stimulated by the addition of TNF-α (25 ng/ml; 24 hours). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033408#s2" target="_blank">Results</a> represent three biological replicates, where variants were tested in triplicate and assayed in duplicate. Statistical analysis with one-way ANOVA shows no significant difference compared with the pCMV control. Error bars represent the standard error of the mean.</p