Expression of NleH-GFP constructs in <i>E. coli</i> O157:H7 grown in defined media.

Abstract

<p>Constructs consisting of 120 bp (pAHE18), 283 bp (pAHE19) or 531 bp (pAHE8) of the NleH1 5′ UTR and 113 bp (pAHE20), 291 bp (pAHE21) or 655 bp (pAHE22) of the NleH2 5′ UTR cloned upstream of <i>gfp</i> were transformed into ZAP193, grown in MEM-HEPES (A) or DMEM (B) and GFP fluorescence measured during growth. All values were corrected for background from a promoter-less GFP (pAJR70) control measured at the same optical density. Graphs represent the average of three experimental repeats.</p