Fluorescence microscopy of NleH-GFP.

Abstract

<p>pAHE8 (NleH1-GFP) and pAHE22 (NleH2-GFP) were transformed in ZAP193, ZAP193Δler and ZAP193ΔgrlA and at OD₆₀₀ = 0.8, dried onto a microscope slide in 4% PFA and stained for EspA filaments. Volocity quantification software was used to determine the average GFP fluorescence per voxel of 100 individual bacteria for NleH1 (A) and NleH2 (B). Each point represents the average GFP fluorescence from a composite from 16 z-slice images thus reducing planar effects. Error bars represent the standard deviation.</p