Study primers and probes.

Abstract

<p>All product sizes are based on the NTHi Rd KW20 genome.</p>*<p>product size varies considerably across strains.</p>**<p><i>hpd</i> probes were labelled with Hex at 5′-end, SpC3 at 3′-end, and Black Hole Quencher (BHQ) at the internal “T”. F (forward primer); R (reverse primer); P (probe); HRM (high resolution melt).</p