Ranking of candidate reference genes in order of their expression stability as calculated by BestKeeper.

Abstract

<p>Note: Expression stability and ranking of 10 reference genes as calculated by Bestkeeper in all samples (A), different ages (B), different tissue types (C), cold-treated (D), heat-treated (E), NaCl-treated (F), PEG-treated (G), ABA-treated (H). Descriptive statistics of 10 candidate genes based on their coefficient of variance (CV) and standard deviation (SD) of Ct values were determined using the whole data set, and all Ct values were analyzed as a total data set. Reference genes are identified as the most stable genes (those with the lowest coefficient of variance and standard deviation; CV±SD).</p