Nonaplex scheme of ITS2 species-diagnostic primers.

Abstract

<p>Representation of the shark nuclear 5.8S and 28S ribosomal RNA genes and ITS2 locus showing relative annealing sites and orientation of primers used in the nonaplex-PCR assay. Shark universal primers (Fish 5.8SF and Fish 28SR) are shown as gray irregular pentagons, while the seven sharpnose species-specific primers are shown by dark gray irregular pentagons. Rlal: <i>Rhizoprionodon lalandei</i>; Rtay: <i>R. taylori</i>; Rolig: <i>R. oligolinx</i>; Rter: <i>R. terranovae</i>; Rlong: <i>R. longurio</i>; Rpor: <i>R. porosus</i>; Racut: <i>R. acutus</i>.</p