A schematic diagram of <i>S. aureus</i> ClfA organization and the insert fragments in the constructs.

Abstract

<p>S: the signal sequence; A: the binding domain; R: the SD repeat region; W: the wall-spanning region; M: the membrane-spanning region. ClfA.RWM: the segment RWM of ClfA from <i>S. aureus</i> Smith Cp which was cloned into pNZ3004. ClfA.SA.rRWM: the inserted portion of pNZ3004-ClfA.SA.rRWM after cloning the segment SA of ClfA from <i>S. aureus</i> Smith Cp cloned into pNZ3004-ClfA.rRWM. ClfA.SAR.rRWM: the inserted portion of pNZ3004-ClfA.SAR.rRWM after cloning the segment SAR of ClfA from <i>S. aureus</i> Smith Cp into pNZ3004-ClfA.rRWM.</p

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