ACC content in barley roots during <i>P. indica</i> colonization.

Abstract

<p>Free (<b>A</b>) and malonylated (<b>B</b>) 1-aminocyclopropane-1-carboxylic acid (ACC) contents were determined in <i>P. indica</i> and mock-treated roots at 1, 3, and 7 days after treatments. At 1 dai, the complete roots were harvested and forwarded to ACC measurements. At 3 and 7 dai, the upper two centimeters (basal part) and the remaining part of the roots (apical part) were analyzed separately. Absolute values are given in nmol • g FW⁻¹ for mock-treated and <i>P. indica</i>-colonized roots. (<b>A</b>) Free ACC levels were significantly enhanced at 3 and 7 dai in the apical zone and 7 dai in the basal part as indicated by Students <i>t</i>-test (* P<0.05, ** P<0.01, *** P<0.001). (<b>B</b>) Malonylated ACC was not significantly altered during <i>P. indica</i> colonization at any timepoint or in any tissue. Data show the mean content of four biological experiments (with at least two technical repetitions per experiment) and bars indicate standard errors.</p