GUS accumulation in roots of <i>ACS1</i>::<i>GUS</i> reporter plants colonized by <i>P. indica</i>.

Abstract

<p><i>Arabidopsis</i> line <i>ACS1</i>::<i>GUS</i> was harvested at 7 dai and, after GUS and WGA-AF 488 staining, analyzed cytologically. (<b>A</b>, <b>B</b>) <i>P. indica</i> colonization at the base of lateral roots (arrows) or primordia (asterisks) of line <i>ACS1</i>::<i>GUS</i> was associated with enhanced GUS accumulation. <i>P. indica</i> (arrowsheads in A) was visualized by staining with WGA-AF 488. (<b>C</b>) In mock-treated <i>ACS1</i>::<i>GUS</i>, GUS staining was weakly detectable e.g. at the lateral root base. Bars = 60 µm.</p