<p>A) Sagittal mouse brain sections were stained with Nissl staining, showing cerebellar atrophy in the <i>nclf</i> brain. B) Autofluorescent storage material was evident in cerebellum, thalamus, hippocampus and cortex of 54 weeks old <i>nclf</i> mice but not in age-matched wild-type controls. The selected areas are shown in panel A (a, b, c, d). Scale bars: 300 µm. C) Ultrastructural analysis showed storage material in cerebellum and hippocampus of the brain of 52 weeks old <i>nclf</i> mice. Scale bar: 2 µm.</p
