Depletion of Cdh1 overcomes ER stress-induced G1 delay.

Abstract

<p>(A) Empty vector-transfected and Cdh1-KD HeLa cells were treated with DMSO or 2.5 µg/ml of TM for 8 h. <i>Left</i>, knockdown efficiency was verified by immunoblotting of endogenous Cdh1. <i>Right</i>, cell cycle distribution of the same cells was analyzed by FACS. Graph quantifies the increase in percentage of cells in G1 after treatment with 2.5 µg/ml of TM for 8 h, normalized to the percentage in DMSO-treated samples. (B) Empty vector-transfected and Cdh1-KD cells were then treated with DMSO or 1 µg/ml of TM for 16 h. <i>Left</i>, knockdown efficiency was verified by immunoblotting of endogenous Cdh1. <i>Right</i>, graph quantifies the increase in percentage of cells in G1 after treatment with 1 µg/ml of TM for 16 h, normalized to the percentage in DMSO-treated samples.</p

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