Effect of integrated spacers on retransformation efficiency.

Abstract

<p>Transformation efficiencies of various PIMs and the control (Wild type <i>E. coli</i> K12 W3110) are given in a logarithmic scale as colony forming units (CFU) per µg of pRSF-1b plasmid DNA. For each PIM, the number of spacers integrated in either CRISPR locus 2.1 or 2.3 is given. All spacers have an AAG PAM, unless indicated otherwise. The exact spacer composition of each PIM is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035888#pone.0035888.s001" target="_blank">Table S1</a>.</p

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