Model of the strand specific positive feedback loop.

Abstract

<p>Cells with a spacer against a known and actively present invader DNA produce targeting Cascade complexes in the expression stage. In the interference stage, Cascade binds the target dsDNA after which the target is cleaved and degraded by Cas3 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035888#pone.0035888-Westra1" target="_blank">[15]</a>. DNA degradation products generated by Cascade and Cas3 (which could be ssDNA or dsDNA) act as precursors for new spacers in the adaptation phase in a strand-specific manner. By integration of these strand-specific precursors, the spacer repertoire against an actively present invader is expanded, completing the positive feedback loop.</p

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