Targeted inactivation of <i>Ilk</i> and loss of ILK transcripts and protein in mouse epidermis.

Abstract

<p>(<b>A</b>) The skin of 3 day-old <i>K14Cre-Ilk^f/+</i> and <i>K14Cre-Ilk^f/f</i> mice (4 each, from two different litters) was isolated and treated with dispase to separate the epidermis from the dermis. Epidermal lysates were prepared and analyzed by immunoblot with antibodies against ILK or γ-tubulin, used as loading control. (<b>B</b>) Total RNA from <i>K14Cre-Ilk^f/+</i> and <i>K14Cre-Ilk^f/f</i> mice (5 each) was used to interrogate GeneChip Mouse Gene 1.0 ST Arrays, and the signal intensity corresponding to individual <i>Ilk</i> exons was analyzed. The average intensity values obtained for each exon in RNA from <i>K14Cre-Ilk^f/f</i> epidermis are shown relative to those in <i>K14Cre-Ilk^f/+</i> tissues, which have been set to 1.0.</p