NF-κB inhibitors block K13-regulated genes.
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Abstract
<p>BCBL1 K13-ER<sup>TAM</sup> cells were treated with two NF–κB inhibitors (2 µM Bay 11-7082 or 2 µM As2O3) for 2 hours followed by 4OHT treatment for additional 24 hours and total RNA was isolated as described in the Materials and Method section. Nine genes were randomly picked and their relative mRNA levels were examined using real-time RT-PCR as explained in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037498#pone-0037498-g002" target="_blank">Figure 2A</a>.</p