<p><b>A.</b> The <i>AMT1</i> locus and gene replacement construct. The <i>AMT1</i> and <i>hph</i> genes are marked with empty and black arrows, respectively. 1F, 2R, 3F, and 4R are primers used to amplify the flanking sequences. <i>Bam</i>HI (B). <b>B.</b> Southern blot analysis with the wild type (PH-1) and Δ<i>amt1</i> transformants (M1, M2, and M3). All DNA samples were digested with <i>Bam</i>HI. The blots were hybridized with probe A (left) amplified with primers AMT1/5F and AMT1/6R and probe B (right) amplified with H852 and H850. <b>C.</b> Colony morphology of the PH-1, Δ<i>amt1</i> mutant M2, and Δ<i>amt1/AMT1</i> transformant C2 cultures grown on CM. Photographs were taken after incubation for 3 days.</p
