SDS PAGE analysis of secreted protein shows that <i>Δdfg5, Δdcw1</i> secretes large amounts of protein.
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Abstract
<p>Samples of secreted protein representing the amount of protein secreted from cells containing 300 µg of cytosolic protein were subjected to SDS PAGE and stained with silver stain. Lane 1, proteins secreted by wild type cells. Lane 2, proteins secreted by <i>Δdcw1</i>. Lane 3, proteins secreted by <i>Δdfg5</i>. Lane 4, proteins secreted by <i>Δdfg5, Δdcw1</i>.</p