Comparison of sequencing results with Ct values from real-time PCR assays.

Abstract

<p>The average Ct values from real-time PCR assays for (A) adenovirus and (B) bocavirus are graphed. Each PCR experiment was repeated at least twice per sample. Samples are grouped from left to right according to whether they were detected by sequencing, not detected by sequencing, or only detected when the number of sequence reads was increased to 21 or 51 million sequences for A and B, respectively.</p