Abstract

<p>Bacteria were harvested in mid-log phase (OD<sub>600 nm</sub> = 0.5), fixed, and prepared as described in Supporting <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002756#s3" target="_blank">Materials and Methods</a> (see <b><a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002756#ppat.1002756.s005" target="_blank">Text S1</a></b>) (<b>A</b>) Representative views of scanning electron microcopy analysis illustrating the morphological alterations (size, form, and cell division abnormalities) due to <i>gbcO</i> inactivation. (<b>B, C</b>) Transmission electron microscopy views of uranyl acetate stained thin cryosections at two magnifications (see scale bars). The presence of the pellicle (electron dense outer layer) at the surface of WT and complemented strains observed at the higher magnification is highlighted with black arrows. An open triangle depicts the equatorial ring (EqR), a zone of active peptidoglycan synthesis seen in almost all WT and complemented cells but absent in the <i>ΔgbcO</i> mutant cells.</p

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