Dermokine is a potential ligand of ClfB.

Abstract

<p>A. Left: Surface plasmon resonance shows the binding of different concentrations of ClfB_(197–542) to synthetic peptide 15 from Dermokine immobilized on a Proteon NLC Sensor Chip. Red, 700 µM; blue, 350 µM; green, 87.5 µM. K_D was found to be 2.37 µM. Right: kinetic and affinity binding values of the ClfB_(197–542) wildtype, S236A or W522A single mutants with Derm15 peptide. B. Comparative close-up view of CK10, Fg α and Derm15 binding to ClfB. The peptides CK10, Fg α and Derm15 are shown as sticks and colored in yellow, slate and green, respectively. The N and C termini are marked. The color schemes of the N2 and N3 domain of ClfB are the same as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002751#ppat-1002751-g001" target="_blank">Figure 1B</a>. C. Closer view of the superimposition of apo-ClfB and ligand-ClfB complexes. N238 and R529 are highlighted and shown as sticks. The apo-ClfB is colored in lime and the others are in the same color scheme as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002751#ppat-1002751-g001" target="_blank">Figure 1B</a>.</p