Blockade of Fibroblast Growth Factor Signaling suppresses VEGF-C expression in 66c14 cancer cells.

Abstract

<p>(A) Left panel, decrease in VEGF-C mRNA expression is detected in FGFR-2DN-expressing 66c14 tumor cells (clones C4, C22) as compared to empty plasmid-transfected group (Control). Right panel, inhibition of VEGF-C protein secretion in FGFR-2DN-expressing 66c14 cell supernatant (C4 and C22) was confirmed by western blotting, and normalized to control group using coomassie blue (C.B.) staining of the membrane as loading control. (B) The FGFR inhibitor PD-173074 inhibits VEGF-C mRNA expression in a dose dependent manner in 66c14 tumor cells. (C) Specific siRNA-mediated inhibition of FGFR-2, but not of FGFR-1, expression reduces VEGF-C mRNA level in 66c14 tumor cells. (*p<0.05 versus respective control group).</p