<i>In vitro</i> lymphangiogenesis is inhibited by FGFR-2DN-expressing 66c14 tumor cells.

Abstract

<p>(<b>A</b>) Co-culture of human lymphatic endothelial cells and mouse mammary 66c14 carcinoma tumor cells induces lymphatic vessel-like tubes in the presence of 66c14 control cells (A1) and FGFR-2DN clone C4-expressing cells in combination with VEGFs (A4) while no vascular structure is observed in FGFR-2DN clone C4 (A2) or clone C22-expressing cells (A3), control cells pretreated with VEGFRs/Fc chimera (A5) or with FGFR inhibitor PD-173074 (A6). (B) Lymphatic tube formation was quantified and expressed as fold change as compared to control condition (A1). (C) <i>In vitro</i> lymphatic tubes formation was induced in the presence of supernatant from control or parental, but not from FGFR-2DN C4 and C22-expressing 66c14 carcinoma cells. (Scale bars, 200 µm in A and C, # and *p<0.05 versus respective control group).</p