Production of reactive oxygen species (ROS) triggered by flg22 and Harpin.
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Abstract
<p>Time-course of ROS accumulation monitored by dihydrorhodamine 123 (DHR 123) in response to the water control (open circles), flg22 (1 µM, closed squares), or Harpin (9 µg<sup>.</sup>ml<sup>−1</sup>, closed triangles) in <i>V. rupestris</i> (<b>A</b>) versus <i>V. vinifera</i> cv. ‘Pinot Noir’ (<b>B</b>). Relative fluorescence recorded at constant exposure time (100 ms) was quantified relative to the respective base fluorescence by the Image J software as described in Material and Methods.</p