Response of the cytoskeleton to flg22.
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Abstract
<p><b>A</b> Disintegration of microtubules visualised by immunofluorescence1 h after addition of 1 µM flg22 or water as negative control. Size bar 20 µm. <b>B</b> Reorganisation of actin filaments visualised by FITC-phalloidin upon flg22 treatment as compared to the water control. Representative geometrical projections from Apotome Z-stacks collected from control (left) or after 3 h (flg22-induced, right) of treatment with 1 µM flg22 are shown. Size bar 20 µm. <b>C</b> Abundance of tyrosinylated α-tubulin in total extracts 24 h after additioin of 1 µM flg22 visualised by Western blotting probing with specific monoclonal antibodies. The same amount of total protein was loaded in each lane, verified by staining of a replicate by Coomassie Brilliant Blue. <b>D</b> Relative abundance of tyrosinylated α-tubulin quantified for the flg22 treatment (flg22, grey bars) as compared to control (con, white bars).</p