ChIP analysis of chromatin modifications.

Abstract

<p><b>A</b>) A schematic drawing of the <i>DKK1</i> gene. Black arrowheads represent primers used for PCR to test three regions in the ChIP assay: promoter, coding and 5′ upstream control. Filled boxes represent exons of the <i>DKK1</i> gene, while the open box represents the <i>DKK1</i> promoter. Black lines represent introns. <b>B</b>) ChIP analysis of the relative protein abundance within different regions of the <i>DKK1</i> gene in SW480 and DLD-1 cells. Immunoprecipitated DNA was analyzed by real time PCR. Specific antibodies used for immunoprecipitation are labeled on the x-axis. A nonspecific rabbit IgG was used as the negative control. Data were plotted as the ratio to the value from 25% of input DNA. Three independent experiments were analyzed and presented as the mean ± SEM. Asterisks (*) indicate statistical significance compared with the Control using the same antibody within the cell line (p<0.05).</p