Pulsed-field gel electrophoresis (PFGE) and schematic representation of BAC clones spanning <i>pFSHα</i> and <i>pFSHβ</i>.

Abstract

<p>(A) Lane 1: λ-<i>Hin</i>dIII marker; lanes 2–5: <i>Not</i>I-digested BAC183O11 DNA at different concentrations (3 µg, 0.2 µg, 1 µg, 0.1 µg); lane 6: MidRange II PFG marker; lanes 7–9: <i>Not</i>I-digested BAC412H8 DNA at different concentrations (0.2 µg, 0.8 µg, 0 µg); upper arrows, linear BAC bands; lower arrows, vector bands. (B–C) BAC clones were mapped by a combination of restriction endonuclease digestion, PFGE, direct DNA sequencing and alignment with the published gene sequence. Solid boxes represent gene exons and dashed lines represent two flanking sequences. The positions of the <i>Not</i>I restriction sites are indicated by the vertical bars.</p