Representative 350 nm HPLC chromatograms of tryptic peptides derived from oxygen-evolving PSII (A–B), OEC-removed (TW) PSII (C–D), and TM (E–F).
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Abstract
<p>In (A), (C), and (E), samples were incubated in the dark at room temperature for two hours (control). In (B), (D), and (F), samples were illuminated with ∼7,000 µmol photons m<sup>−2</sup> s<sup>−1</sup> of white light for two hours at 25°C. Fraction A is filled with horizontal stripes, and fraction C is filled with dots. The chromatograms are displaced on the y-axis for presentation purposes. The tick increments are 0.085 A.U. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042220#pone.0042220.s002" target="_blank">Table S1</a> for average retention times and summary of light-induced changes. Fraction C corresponds to fraction 1 in ref <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042220#pone.0042220-Dreaden1" target="_blank">[19]</a>.</p